The Indication of Biofilms and Non-Cultivated Microorganisms while Monitoring the Biological Safety of Food Raw Materials

The relevance of the study and the presence of gaps in existing knowledge on the topic. Monitoring the biosafety of food raw materials by microbiological indicators is an urgent problem due to an increase in the number foodborne infections worldwide.

The aim of the work is to study the features of the formation of biofilms and uncultured microorganisms under various cultivation conditions

Methods. Morphometric and densitometric indicators of biofilms and uncultured microorganisms were studied under various cultivation conditions. To study the growth and development populations of microorganisms, media containing growth factors for cell wall repair and L-shape reversal of microorganisms were used.

Results and discussion. Microbiological control of critical points in animal husbandry technology and food production has examined the species composition and etiological significance virulence factors of strains producing adhesive antigens, bacteriocins, hemolysins, toxins, extended-spectrum p-lactamases, which determine the tendency to increase multidrug resistance. The morphological and functional features of biofilms, which are communities of microorganisms that secrete the polymer matrix and adhere to tissues of susceptible animal species and abiotic surfaces of livestock buildings and food production, were studied. Direct correlative relationships between filamentation, dispersion multi-species biofilms of microorganisms and the development dystrophic and necrotic processes in the tissues and organs mammals and birds have been established. To optimize the microbiological diagnosis of infectious diseases, effective methods for detecting heteromorphic biofilms and uncultured microorganisms have been tested and selected. To prevent the formation biofilms of pathogenic microorganisms, drugs that reduce the level of microbiological parameters primary contamination are promising; minimize adhesive properties, as well as biocides that destroy the intercellular matrix.

Conclusions. The ability to form biofilms, the variability of phenotypic characters, the multiplicity of virulence factors, the emergence of resistant forms of bacteria due to the synthesis exopolysaccharides, significantly reduce the effectiveness antiepizootic and diagnostic measures. The development of accelerated methods for the detection of biofilms and the differentiation of uncultivated microorganisms will make it possible to scientifically substantiate and develop a set of measures aimed at preventing animal diseases and obtaining safe livestock products in order to prevent human diseases.

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