The Effectiveness of Immunodiagnostic Studies in Tissue Parasitoses of Pigs

It is well known that pig farming is one of the most profitable branches of animal husbandry and is able to increase meat production in a short time with low feed costs per unit of production. That is why industrial complexes and small commodity farms for raising pigs are created and operate. However, the presence of tissue helminthiasis in the body of live animals and pork significantly affects the efficiency of fattening, the quality of meat and the biological safety of products. Approximately 2 to 5% of pig carcasses are registered as affected by tissue parasites. The Russian Federation contains about 25 million pigs, so we can assume that more than 1 million pigs are sick, i.e. they are ineffectively fattened and are a source of pathogens that are dangerous to human health. At the same time, there are methods of lifetime diagnostics of swine parasitosis and their application could significantly increase the economic efficiency of the pig industry and improve the epidemic situation for helminthiasis in all regions of the country. The purpose of the study was to develop a test system based on ELISA and evaluate its effectiveness on slaughtered animals in some regions of the Central Federal District. The parameters of the test system were tested on 96 well plates, and full extracts of water-salt solutions of parasites – E. granulosus, T. spiralis, C. cellulosae, Sarcocystis spp-were used as the antigen. Blood serums were delivered from meat processing plants where pigs were slaughtered and were accompanied by a Protocol of veterinary and sanitary examination. Of the 550 serums tested 43 had antibodies i.e. they gave a positive response in a multi-antigen test. According to the examination report, 25 carcasses infected with tissue parasites were registered.

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