Обзор современного состояния и перспективы биотехнологии олеогенных дрожжей
https://doi.org/10.36107/hfb.2026.i1.s299
Аннотация
Переход к возобновляемым ресурсам и микробным технологиям может повысить устойчивость продовольственной системы. Микробные масла обладают высокой пищевой ценностью и экологическими преимуществами по сравнению с традиционными растительными и животными жирами.
Цель. Обобщить современные данные об использовании олеогенных дрожжей для получения микробного масла и оценить перспективы их биотехнологического применения.
Материалы и методы. Произведен целенаправленный поиск научных публикаций за 2018–2025 гг. в базах Web of Science, Scopus, PubMed, Google Scholar и eLibrary, по ключевым словам, на английском и русском языках: «oleaginous yeasts», «microbial oil», «жирные кислоты дрожжей» и др. В обзор включены 80 источников, англоязычные статьи из рецензируемых журналов, соответствующие теме исследования.
Результаты и обсуждение. Рассмотрены тенденции мирового рынка растительных, животных и микробных масел. Проанализированы основные группы микроорганизмов-продуцентов липидов, особенно дрожжи, их липидный профиль и методы повышения выхода микробного масла (мутагенез, генная инженерия). Обсуждены биотехнологические применения олеогенных дрожжей в производстве биодизеля, аналогов пищевых масел и кормовых добавок. Выводы. Олеогенные дрожжи представляют перспективный источник жиров, способный диверсифицировать сырьевую базу пищевой и топливной промышленности. Требуется дальнейшее совершенствование методов селекции штаммов и технологий культивирования для повышения эффективности производства микробных масел.
Ключевые слова
Об авторах
Иван Дмитриевич БельскийРоссия
Аспирант кафедры «Биотехнологии и биоорганического синтеза»
Александра Игоревна Карачун
Россия
Магистр кафедры «Биотехнологии и биоорганического синтеза».
SPIN-код: 6591-1303
Иван Андреевич Фоменко
Россия
Кандидат технических наук, доцент кафедры «Биотехнологии и биоорганического синтеза»
SPIN-код: 5861-2838
Денис Игоревич Алексаночкин
Россия
Аспирант кафедры «Биотехнологии и биоорганического синтеза», Инженер-исследователь Лаборатории фундаментальных и прикладных исследований качества и технологий пищевых продуктов (ПНИЛ биотехнологии) / Центр коллективного пользования научным оборудованием "Качество и безопасность пищевых продуктов". SPIN-код: 1732-9580
Алена Сергеевна Шомахова
Россия
Студент кафедры «Биотехнологии и биоорганического синтеза»
Наталья Геннадьевна Машенцева
Россия
Доктор технических наук, профессор кафедры «Биотехнологии и биоорганического синтеза», профессор РАН
SPIN-код: 9791-5806
Список литературы
1. Хвостов, И. И., & Борисова, А. В. (2021). Анализ технологий получения микробных масел. Ползуновский вестник, (3), 83-88. https://doi.org/10.25712/ASTU.2072-8921.2021.03.011
2. Khvostov, I. I. & Borisova, A. V. (2021). Biosynthesis of oils. Polzunovskiy vestnik, (3), 83-88. (In Russ.). https://doi.org/10.25712/ASTU.2072-8921.2021.03.011
3. Abdelkarim, O. H., Wijffels, R. H., & Barbosa, M. J. (2025). Microalgal lipid production: A comparative analysis of Nannochloropsis and Microchloropsis strains. Journal of Applied Phycology, 37(1), 15–34. https://doi.org/10.1007/s10811-024-03318-7
4. Abeln, F., & Chuck, C. J. (2019). Achieving a high‐density oleaginous yeast culture: Comparison of four processing strategies using Metschnikowia pulcherrima. Biotechnology and Bioengineering, 116(12), 3200–3214. https://doi.org/10.1002/bit.27141
5. Abeln, F., & Chuck, C. J. (2021). The history, state of the art and future prospects for oleaginous yeast research. Microbial Cell Factories, 20(1), 221. https://doi.org/10.1186/s12934-021-01712-1
6. Adiguzel, A. O. (2023). The use of omics technologies, random mutagenesis, and genetic transformation techniques to improve algae for biodiesel industry. Technological advancement in algal biofuels production. Clean Energy Production Technologies. Springer Nature, 43–80. https://doi.org/10.1007/978-981-19-6806-8_2
7. Agboola, J. O., Øverland, M., Skrede, A., & Hansen, J. Ø. (2021). Yeast as major protein‐rich ingredient in aquafeeds: A review of the implications for aquaculture production. Reviews in Aquaculture, 13(2), 949–970. https://doi.org/10.1111/raq.12507
8. Andreo-Martinez, P., Ortiz-Martinez, V. M., Salar-Garcia, M. J., Veiga-del-Bano, J. M., Chica, A., & Quesada-Medina, J. (2022). Waste animal fats as feedstock for biodiesel production using non-catalytic supercritical alcohol transesterification: A perspective by the PRISMA methodology. Energy for Sustainable Development, 69, 150–163. https://doi.org/10.1016/j.esd.2022.06.004
9. Arous, F., Azabou, S., Triantaphyllidou, I. E., Aggelis, G., Jaouani, A., Nasri, M., & Mechichi, T. (2017). Newly isolated yeasts from Tunisian microhabitats: Lipid accumulation and fatty acid composition. Engineering in Life Sciences, 17(3), 226–236. https://doi.org/10.1002/elsc.201500156
10. Arous, F., Mechichi, T., Nasri, M., & Aggelis, G. (2016). Fatty acid biosynthesis during the life cycle of Debaryomyces etchellsii. Microbiology, 162(7), 1080–1090. https://doi.org/10.1099/mic.0.000298
11. Arora, N., Pienkos, P. T., Pruthi, V., Poluri, K. M., & Guarnieri, M. T. (2020). Leveraging algal omics to reveal potential targets for augmenting TAG accumulation. Biotechnology Advances, 36(4), 1274–1292. https://doi.org/10.1016/j.biotechadv.2018.04.005
12. Arora, N., Yen, H. W., & Philippidis, G. P. (2020). Harnessing the power of mutagenesis and adaptive laboratory evolution for high lipid production by oleaginous microalgae and yeasts. Sustainability, 12(12), 5125. https://doi.org/10.3390/su12125125
13. Beopoulos, A., Cescut, J., Haddouche, R., Uribelarrea, J. L., Molina-Jouve, C., & Nicaud, J. M. (2009). Yarrowia lipolytica as a model for bio-oil production. Progress in Lipid Research, 48(6), 375–387. https://doi.org/10.1016/j.plipres.2009.08.005
14. Beopoulos, A., Nicaud, J. M., & Gaillardin, C. (2011). An overview of lipid metabolism in yeasts and its impact on biotechnological processes. Applied Microbiology and Biotechnology, 90(4), 1193–1206. https://doi.org/10.1007/s00253-011-3212-8
15. Beld, J., Lee, D. J., & Burkart, M. D. (2015). Fatty acid biosynthesis revisited: Structure elucidation and metabolic engineering. Molecular BioSystems, 11(1), 38–59. https://doi.org/10.1039/c4mb00443d
16. Bhuiya, M. M. K., Rasul, M. G., Khan, M. M. K., Ashwath, N., Azad, A. K., & Hazrat, M. A. (2014). Second generation biodiesel: Potential alternative to edible oil-derived biodiesel. Energy Procedia, 61, 1969–1972. https://doi.org/10.1016/j.egypro.2014.12.054
17. Bleisch, R., Freitag, L., Ihadjadene, Y., Sprenger, U., Steingröwer, J., Walther, T., & Krujatz, F. (2022). Strain development in microalgal biotechnology – random mutagenesis techniques. Life, 12(7), 961. https://doi.org/10.3390/life12070961
18. Braunwald, T., French, W. T., Claupein, W., & Graeff-Hönninger, S. (2016). Economic assessment of microbial biodiesel production using heterotrophic yeasts. International Journal of Green Energy, 13(3), 274–282. https://doi.org/10.1080/15435075.2014.940957
19. Cao, X., Pan, Y., Wei, W., Yuan, T., Wang, S., Xiang, L., & Yuan, Y. (2021). Single cell oil production by Trichosporon sp.: Effects of fermentation conditions on fatty acid composition and applications in synthesis of structured triacylglycerols. LWT - Food Science and Technology, 148, 111691. https://doi.org/10.1016/j.lwt.2021.111691
20. Caporusso, A., Capece, A., & De Bari, I. (2021). Oleaginous yeasts as cell factories for the sustainable production of microbial lipids by the valorization of agri-food wastes. Fermentation, 7(2), 50. https://doi.org/10.3390/fermentation7020050
21. Chaturvedi, S., Bhattacharya, A., & Khare, S. K. (2018). Trends in oil production from oleaginous yeast using biomass: Biotechnological potential and constraints. Applied Biochemistry and Microbiology, 54(4), 361–369. https://doi.org/10.1134/S000368381804004X
22. Chatzifragkou, A., Makri, A., Belka, A., Bellou, S., Mavrou, M., Mastoridou, M., & Papanikolaou, S. (2011). Biotechnological conversions of biodiesel derived waste glycerol by yeast and fungal species. Energy, 36(2), 1097–1108. https://doi.org/10.1016/j.energy.2010.11.040
23. Dhanavel, N., & Nandakrishnan, M. H. (2024). A review of animal fat: a great source for industrial applications. Journal of Chemical Review, 6(2), 115-137. https://doi.org/10.48309/JCR.2024.425819.1276
24. Galafassi, S., Cucchetti, D., Pizza, F., Franzosi, G., Bianchi, D., & Compagno, C. (2012). Lipid production for second generation biodiesel by the oleaginous yeast Rhodotorula graminis. Bioresource Technology, 111, 398–403. https://doi.org/10.1016/j.biortech.2012.02.004
25. Galanakis, C. M. (2024). The future of food. Foods, 13(4), 506. https://doi.org/10.3390/foods13040506
26. Gong, Z., Shen, H., Zhou, W., Wang, Y., Yang, X., & Zhao, Z. K. (2015). Efficient conversion of acetate into lipids by the oleaginous yeast Cryptococcus curvatus. Biotechnology for Biofuels, 8(1), 189. https://doi.org/10.1186/s13068-015-0371-3
27. Gong, Z., Wang, Q., Shen, H., Hu, C., Jin, G., & Zhao, Z. K. (2012). Co-fermentation of cellobiose and xylose by Lipomyces starkeyi for lipid production. Bioresource Technology, 117, 20–24. https://doi.org/10.1016/j.biortech.2012.04.063
28. Gouda, M. K., Omar, S. H., & Aouad, L. M. (2008). Single cell oil production by Gordonia sp. DG using agro-industrial wastes. World Journal of Microbiology and Biotechnology, 24(9), 1703–1711. https://doi.org/10.1007/s11274-008-9664-z
29. Hájek, M., Vávra, A., de Paz Carmona, H., & Kocík, J. (2021). The catalysed transformation of vegetable oils or animal fats to biofuels and bio-lubricants: A review. Catalysts, 11(9), 1118. https://doi.org/10.3390/catal11091118
30. Keskin, A., Ünlü, A. E., & Takac, S. (2023). Utilization of olive mill wastewater for selective production of lipids and carotenoids by Rhodotorula glutinis. Applied Microbiology and Biotechnology, 107(15), 4973–4985. https://doi.org/10.1007/s00253-023-12625-x
31. Kumar, M., Rathour, R., Gupta, J., Pandey, A., Gnansounou, E., & Thakur, I. S. (2020). Bacterial production of fatty acid and biodiesel: Opportunity and challenges. Refining biomass residues for sustainable energy and bioproducts. Technology, Advances, Life Cycle Assessment, and Economics. Academic Press, 21–49. https://doi.org/10.1016/B978-0-12-818996-2.00002-8
32. Kurosawa, K., Wewetzer, S. J., & Sinskey, A. J. (2013). Engineering xylose metabolism in triacylglycerol-producing Rhodococcus opacus for lignocellulosic fuel production. Biotechnology for Biofuels, 6(1), 134. https://doi.org/10.1186/1754-6834-6-134
33. Lau, Z., Stuart, D., & McNeil, B. (2019). Establishing CRISPR/Cas9 in Lipomyces starkeyi. Alberta Academic Review, 2(2), 51–52. https://doi.org/10.29173/aar61
34. Li, X., Xiong, L., Chen, X., Huang, C., Chen, X., & Ma, L. (2015). Effects of acetic acid on growth and lipid production by Cryptococcus albidus. Journal of the American Oil Chemists' Society, 92(8), 1113–1118. https://doi.org/10.1007/s11746-015-2685-5
35. Lin, X., Wu, J., & Li, Z. (2025). Vegetable oil intake: The distinctive trilateral relationship of bile acid, gut microbiota and health. Trends in Food Science & Technology, 160, 105001. https://doi.org/10.1016/j.tifs.2025.105001
36. Liu, B., & Zhao, Z. (2007). Biodiesel production by direct methanolysis of oleaginous microbial biomass. Journal of Chemical Technology & Biotechnology, 82(8), 775–780. https://doi.org/10.1002/jctb.1744
37. Liu, J., Liu, J. N., Yuan, M., Shen, Z. H., Peng, K. M., Lu, L. J., & Huang, X. F. (2016). Bioconversion of volatile fatty acids derived from waste activated sludge into lipids by Cryptococcus curvatus. Bioresource Technology, 211, 548–555. https://doi.org/10.1016/j.biortech.2016.03.146
38. Louhasakul, Y., Cheirsilp, B., Maneerat, S., & Prasertsan, P. (2018). Direct transesterification of oleaginous yeast lipids into biodiesel: Development of vigorously stirred tank reactor and process optimization. Biochemical Engineering Journal, 137, 232–238. https://doi.org/10.1016/j.bej.2018.06.009
39. Maltsev, Y., & Maltseva, K. (2021). Fatty acids of microalgae: Diversity and applications. Reviews in Environmental Science and Bio/Technology, 20(2), 515–547. https://doi.org/10.1007/s11157-021-09571-3
40. Martinez-Silveira, A., Pereyra, V., Garmendia, G., Rufo, C., & Vero, S. (2019). Optimization of culture conditions of Rhodotorula graminis S1/2R to obtain saponifiable lipids for the production of second-generation biodiesel. Environmental Sustainability, 2(4), 419–428. https://doi.org/10.1007/s42398-019-00085-x
41. McNeil, B. A., & Stuart, D. T. (2018). Lipomyces starkeyi: An emerging cell factory for production of lipids, oleochemicals and biotechnology applications. World Journal of Microbiology and Biotechnology, 34(10), 147. https://doi.org/10.1007/s11274-018-2532-6
42. Mielke, T. (2017). World markets for vegetable oils and animal fats: Dynamics of global production, trade flows, consumption and prices. Biokerosene: Status and prospects. Springer, 147–188. https://doi.org/10.1007/978-3-662-53065-8_8
43. Murphy, D. J. (2025). Agronomy and environmental sustainability of the four major global vegetable oil crops: Oil palm, soybean, rapeseed, and sunflower. Agronomy, 15(6), 1465. https://doi.org/10.3390/agronomy15061465
44. Naylor, R. L., & Higgins, M. M. (2017). The political economy of biodiesel in an era of low oil prices. Renewable and Sustainable Energy Reviews, 77, 695–705. https://doi.org/10.1016/j.rser.2017.04.026
45. Němcová, A., Szotkowski, M., Samek, O., Cagáňová, L., Sipiczki, M., & Márová, I. (2021). Use of waste substrates for the lipid production by yeasts of the genus Metschnikowia-Screening study. Microorganisms, 9(11), 2295. https://doi.org/10.3390/microorganisms9112295
46. Niehus, X., Casas-Godoy, L., Vargas-Sánchez, M., & Sandoval, G. (2018). A fast and simple qualitative method for screening oleaginous yeasts on agar. Journal of Lipids, 2018(1), 5325804. https://doi.org/10.1155/2018/5325804
47. Papanikolaou, S., & Aggelis, G. (2011). Lipids of oleaginous yeasts. Part I: Biochemistry of single cell oil production. European Journal of Lipid Science and Technology, 113(8), 1031–1051. https://doi.org/10.1002/ejlt.201100014
48. Parker, L., Ward, K., Pilarski, T., Price, J., Derkach, P., Correa, M., & Franklin, S. (2024). Development and large-scale production of high-oleic acid oil by fermentation of microalgae. Fermentation, 10(11), 566. https://doi.org/10.3390/fermentation10110566
49. Parsons, S., Abeln, F., McManus, M. C., & Chuck, C. J. (2019). Techno‐economic analysis (TEA) of microbial oil production from waste resources as part of a biorefinery concept: Assessment at multiple scales under uncertainty. Journal of Chemical Technology & Biotechnology, 94(3), 701–711. https://doi.org/10.1002/jctb.5811
50. Passoth, V., & Sandgren, M. (2019). Biofuel production from straw hydrolysates: Current achievements and perspectives. Applied Microbiology and Biotechnology, 103(13), 5105–5116. https://doi.org/10.1007/s00253-019-09863-3
51. Patel, A., Karageorgou, D., Rova, E., Katapodis, P., Rova, U., & Christakopoulos, P. (2020). An overview of potential oleaginous microorganisms and their role in biodiesel and omega-3 fatty acid-based industries. Microorganisms, 8(3), 434. https://doi.org/10.3390/microorganisms8030434
52. Pérez-Rodríguez, A., Flores-Ortiz, C. M., Chávez-Camarillo, G. M., Cristiani-Urbina, E., & Morales-Barrera, L. (2023). Potential capacity of Candida wangnamkhiaoensis to produce oleic acid. Fermentation, 9(5), 443. https://doi.org/10.3390/fermentation9050443
53. Ramírez-Castrillón, M., Jaramillo-Garcia, V. P., Rosa, P. D., Landell, M. F., Vu, D., Fabricio, M. F., & Valente, P. (2017). The oleaginous yeast Meyerozyma guilliermondii BI281A as a new potential biodiesel feedstock: Selection and lipid production optimization. Frontiers in Microbiology, 8, 1776. https://doi.org/10.3389/fmicb.2017.01776
54. Ratledge, C. (2013). Single cell oils for the 21st century. Single cell oils. Microbial and Algal Oils. AOCS Press, 3–26. https://doi.org/10.1016/B978-1-893997-73-8.50005-0
55. Rees, P., Summers, H. D., Filby, A., Carpenter, A. E., & Doan, M. (2022). Imaging flow cytometry. Nature Reviews Methods Primers, 2(1), 86. https://doi.org/10.1038/s43586-022-00167-x
56. Robinson, J. P., Ostafe, R., Iyengar, S. N., Rajwa, B., Fischer, R., & Walther, T. (2023). Flow cytometry: The next revolution. Cells, 12(14), 1875. https://doi.org/10.3390/cells12141875
57. Santamauro, F., Whiffin, F. M., Scott, R. J., & Chuck, C. J. (2014). Low-cost lipid production by an oleaginous yeast cultured in non-sterile conditions using model waste resources. Biotechnology for Biofuels, 7(1), 34. https://doi.org/10.1186/1754-6834-7-34
58. Sabahannur, S., & Alimuddin, S. (2022). Identification of fatty acids in virgin coconut oil (VCO), cocoa beans, crude palm oil (CPO), and palm kernel beans using gas chromatography. In IOP Conference Series: Earth and Environmental Science, 1083(1), 012036. https://doi.org/10.1088/1755-1315/1083/1/012036
59. Sekova, V. Y., Isakova, E. P., & Deryabina, Y. I. (2015). Biotechnological applications of the extremophilic yeast Yarrowia lipolytica. Applied Biochemistry and Microbiology, 51(3), 278–291. https://doi.org/10.1134/S0003683815030151
60. Soccol, C. R., Colonia, B. S. O., de Melo Pereira, G. V., Mamani, L. D. G., Karp, S. G., Soccol, V. T., & de Carvalho, J. C. (2022). Bioprospecting lipid-producing microorganisms: From metagenomic-assisted isolation techniques to industrial application and innovations. Bioresource Technology, 346, 126455. https://doi.org/10.1016/j.biortech.2021.126455
61. Spagnuolo, M., Yaguchi, A., & Blenner, M. (2019). Oleaginous yeast for biofuel and oleochemical production. Current Opinion in Biotechnology, 57, 73–81. https://doi.org/10.1016/j.copbio.2019.02.011
62. Spier, F., Buffon, J. G., & Burkert, C. A. (2015). Bioconversion of raw glycerol generated from the synthesis of biodiesel by different oleaginous yeasts: Lipid content and fatty acid profile of biomass. Indian Journal of Microbiology, 55(4), 415–422. https://doi.org/10.1007/s12088-015-0533-9
63. Stuhr, N. L., Nhan, J. D., Hammerquist, A. M., Van Camp, B., Reoyo, D., & Curran, S. P. (2022). Rapid lipid quantification in Caenorhabditis elegans by oil red O and Nile red staining. Bio-protocol, 12(5), e4340. https://doi.org/10.21769/BioProtoc.4340
64. Szczepańska, P., Hapeta, P., & Lazar, Z. (2021). Advances in production of high-value lipids by oleaginous yeasts. Critical Reviews in Biotechnology, 42(1), 1–22. https://doi.org/10.1080/07388551.2021.1922353
65. Tsakraklides, V., Kamineni, A., Consiglio, A. L., MacEwen, K., Friedlander, J., Blitzblau, H. G., & Brevnova, E. E. (2018). High-oleate yeast oil without polyunsaturated fatty acids. Biotechnology for Biofuels, 11(1), 131. https://doi.org/10.1186/s13068-018-1131-y
66. Wang, C., Li, Z., & Wu, W. (2023). Understanding fatty acid composition and lipid profile of rapeseed oil in response to nitrogen management strategies. Food Research International, 165, 112565. https://doi.org/10.1016/j.foodres.2023.112565
67. Wang, X., Ma, L., Yan, S., Chen, X., & Growe, A. (2023). Trade for food security: The stability of global agricultural trade networks. Foods, 12(2), 271. https://doi.org/10.3390/foods12020271
68. Wen, Z., & Al Makishah, N. H. (2022). Recent advances in genetic technology development of oleaginous yeasts. Applied Microbiology and Biotechnology, 106(17), 5385–5397. https://doi.org/10.1007/s00253-022-12101-y
69. Yang, X., Jin, G., Gong, Z., Shen, H., Bai, F., & Zhao, Z. K. (2014). Recycling biodiesel-derived glycerol by the oleaginous yeast Rhodosporidium toruloides Y4 through the two-stage lipid production process. Biochemical Engineering Journal, 91, 86–91. https://doi.org/10.1016/j.bej.2014.07.015
70. Zhang, S., Skerker, J. M., Rutter, C. D., Maurer, M. J., Arkin, A. P., & Rao, C. V. (2016). Engineering Rhodosporidium toruloides for increased lipid production. Biotechnology and Bioengineering, 113(5), 1056–1066. https://doi.org/10.1002/bit.25864
71. Zhang, X. Y., Li, B., Huang, B. C., Wang, F. B., Zhang, Y. Q., Zhao, S. G., & Wang, Z. P. (2022). Production, biosynthesis, and commercial applications of fatty acids from oleaginous fungi. Frontiers in Nutrition, 9, 873657. https://doi.org/10.3389/fnut.2022.873657
72. Zheng, S., Sun, S., Zou, S., Song, J., Hua, L., Chen, H., & Wang, Q. (2024). Effects of culture temperature and light regimes on biomass and lipid accumulation of Chlamydomonas reinhardtii under carbon-rich and nitrogen-limited conditions. Bioresource Technology, 399, 130613. https://doi.org/10.1016/j.biortech.2024.130613
Рецензия
Для цитирования:
Бельский И.Д., Карачун А.И., Фоменко И.А., Алексаночкин Д.И., Шомахова А.С., Машенцева Н.Г. Обзор современного состояния и перспективы биотехнологии олеогенных дрожжей. Health, Food & Biotechnology. 2026;8(1):41-59. https://doi.org/10.36107/hfb.2026.i1.s299
For citation:
Belsky I.D., Karachun A.I., Fomenko I.A., Aleksanochkin D.I., Shomakhova A.S., Mashentseva N.G. Review of the Current State and Prospects of Oleaginous Yeasts Biotechnology (Systematic Scoping Review). Health, Food & Biotechnology. 2026;8(1):41-59. (In Russ.) https://doi.org/10.36107/hfb.2026.i1.s299
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