Research of antioxidant properties α-tocopherol, quercetin, β-sitosterol, retinyl palmitate and development of an antioxidant complex based on them

The  antioxidant  properties  of  individual  substances  and  their  mixtures,  such  as α-tocopherol,  quercetin, β-sitosterol,  retinyl  palmitate,  were  studied  to  create  a  multiantioxidant composition that stabilizes emulsion systems. To determine the antioxidant activity,  the  ability  of  individual  compounds  and  their  mixtures  to  inhibit  the  thermal oxidation  of  oleic  acid  methyl  ester  was  evaluated.  It  is  shown  that  an  increase  in  the antioxidant properties of a binary mixture of vitamins is observed with an increase in the concentration of α-tocopherol and a decrease in the concentration of retinyl palmitate. The effect of mutual suppression of the antioxidant properties of a mixture of retinyl palmitate and β-sitosterol was established. It is proved that the value of the antioxidant activity of the  reaction  mixture,  including  quercetin  in  combination  with  vitamins  or β-sitosterin, has  additive  properties.  A  mixture  consisting  of  4  components: α-tocopherol,  quercetin, β-sitosterol,  retinyl  palmitate  is  characterized  by  an  increase  in  antioxidant  properties by  2.5  times  at  minimum  concentrations,  and  by  1.5  times  at  maximum  concentrations. It  was  found  that  all  the  studied  natural  compounds  had  antiperoxide  activity,  while the  highest  level  was  observed  in  quercetin,  then  in  retinyl  palmitate, β-sitosterol  and α-tocopherol. It was shown that the mixture of natural antioxidants had an antiperoxide activity 1.5 times higher than that of quercetin. Based on the obtained regression equation that  adequately  describes  the  process,  an  optimization  program  was  implemented  that allowed the formation of an antioxidant complex including retinyl palmitate, α-tocopherol, β-sitosterol and quercetin in optimal ratios. It is shown that the antioxidant activity of the complex was 1.7 times higher than the result of this indicator in a mixture consisting of 4 studied components. The developed d\antioxidant complex is recommended for use in products with an emulsion base.

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