Obtaining a Cell Culture of Ginger
https://doi.org/10.36107/hfb.2019.i4.s280
Abstract
Ginger, as a spice and medicinal plant from ancient times to the present, is in great demand in India and China due to the wide range of effects on the human body. In the wild, ginger does not grow by itself, therefore it is grown on special plantations covering large areas on an industrial scale. Climatic conditions are also important for active plant growth. Standard ginger breeding programs are difficult due to low productivity and lack of natural seed quantity. The biotechnological method of cultivating plant cells makes it possible to obtain an unlimited number of identical cells in 3 to 4 weeks. Zingiber sp. rhizomes, stems and leaves are served as the starting material for research and obtaining callus cell cultures. To obtain primary callus for their further cultivation, various modifications of the Murashige and Skoog medium, as well as the Hamburg medium, were used. Explants were cultured in the dark at 26 ° C and a humidity of 60–70% . The growing cycle was 25–30 days. According to the results of the analysis, new data on the properties of Zingiber plant cell cultures was obtained, ways to solve a number of global environmental problems were proposed. As a result of using the classical method of obtaining a callus culture of a sterile intact plant was obtained a new initial ginger cell strain in vitro with stable growth characteristics.
About the Authors
L. A. Ivanova
Moscow State University of Food Production
Russian Federation
Russian Federation
Lyudmila A. Ivanova
11 Volokolamskoe highway, Moscow, 125080, Russian Federation
I. A. Fomenko
Moscow State University of Food Production; JSC “Biokhimmash”
Russian Federation
Russian Federation
Ivan A. Fomenko
11 Volokolamskoe highway, Moscow, 125080, Russian Federation
4 Klara Cetkin str., Moscow, 127299, Russian Federation
V. V. Fomenko
JSC “Biokhimmash”
Russian Federation
Russian Federation
Viktoriya V. Fomenko
Klara Cetkin str., Moscow, 127299, Russian Federation
L. A. Churmasova
Moscow State University of Food Production
Russian Federation
Russian Federation
Lyudmila A. Churmasova
11 Volokolamskoe highway, Moscow, 125080, Russian Federation
A. M. Shhennikova
Moscow State University of Food Production
Russian Federation
Russian Federation
Anastasiya M. Shhennikova
11 Volokolamskoe highway, Moscow, 125080, Russian Federation
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Review
For citations:
Ivanova L.A., Fomenko I.A., Fomenko V.V., Churmasova L.A., Shhennikova A.M. Obtaining a Cell Culture of Ginger. Health, Food & Biotechnology. 2019;1(4):92-104. (In Russ.) https://doi.org/10.36107/hfb.2019.i4.s280
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