Introduction: Data openness policies, such as the Linking Data Policy and Sharing Data Policy, are essential tools for promoting the principles of open science. They enhance the transparency, reproducibility, and accessibility of scientific data, which is particularly relevant in the context of growing interest in open science and the need to address global challenges.

Purpose: To analyze data openness policies, identify their advantages and challenges, and develop recommendations for their implementation.

Materials and Methods: The study was based on an analysis of editorial policies and author guidelines from leading journals, as well as the principles of FAIR (Findable, Accessible, Interoperable, Reusable). Examples of successful implementation of data openness policies were reviewed, and key challenges associated with their adoption were identified.

Results: Data openness policies enhance the transparency and reproducibility of research, accelerate scientific discoveries, and strengthen trust in scientific results. However, their implementation faces several challenges, including a lack of skills among researchers, data confidentiality issues, and the need for significant resources. Based on the analysis, recommendations for implementing such policies were developed, including the creation of author guidelines, the selection of repositories, integration with manuscript submission systems, and researcher training.

Conclusion: The adoption of Linking Data Policy and Sharing Data Policy represents an important step toward open science but requires careful planning and consideration of various aspects. While the benefits of these policies are evident, their implementation involves significant challenges. Successful adoption necessitates providing training for researchers, collaborating with repositories, developing standards, and offering adequate support.

Introduction. Diseases of the circulatory system (DCS) are one of the most pressing healthcare issues, with a significant role in preventing and slowing the progression of DCS attributed to prevention, including through dietary adjustments and addressing nutrient deficiencies.Purpose. To conduct an open, prospective, cohort, uncontrolled study involving a sample of 100 patients with a 3-month observation period, during which the effectiveness and tolerability of using "AD Medicine" colloidal phytoformulas for the prevention of DCS were evaluated, along with their impact on vascular blood flow and the condition of cerebral and lower extremity vessels.Materials and Methods. 100 patients were observed over a 3-month period while using "AD Medicine" colloidal phytoformulas (Brain Booster, Reoton Complex, Anti-Oxidant) for the prevention of DCS. The evaluation criteria included hemodynamic parameters, lipid profile indicators, the condition of cerebral and lower extremity vessels, as well as subjective quality of life assessments by study participants and tolerability profiles.Results and Conclusions. The study provided evidence of the effectiveness of the combined use of the following colloidal phytoformulas: "Brain Booster" "Rheoton Complex" and "Anti-Oxidant" (manufacturer – "AD Medicine", Russia).Regarding the impact on cerebral vessels: improvement in cerebral blood supply by 63-66%, vascular tone by 55-61%, peripheral resistance by 58%, vascular elasticity by 38%, and venous outflow by 62-67%.Regarding the impact on blood flow and the condition of lower extremity vessels: the most pronounced effect was observed in peripheral vascular resistance (57.3%, p<0.001); improvement in several parameters (volumetric pulse blood supply, vascular wall elasticity, venous outflow) was approximately 40%; vascular tone and blood flow velocity in the lower extremity vessels showed lesser changes (24.8% and 32.6%, respectively).Regarding the impact on hemodynamic and lipid metabolism parameters, the changes were minimal: the variation in indicators did not exceed 5% and cannot be considered clinically significant.

Introduction. NADP-oxidase 4 or NOX4 is an enzyme of the oxidase group that plays a significant role in the pathogenesis of ischemic stroke. First of all, NOX4 mediates the activation of oxidative stress and mitochondrial dysfunction, which makes it a promising pharmacotherapeutic target.

Purpose. To evaluate the effect of the administration of 3-[(1E)-3-(3,4-dimethylphenyl)-3-oxoprope-1-en-1-yl]-4H-1-benzopyran-4-one on the change in NOX4-dependent mitochondrial biogenesis in rats with focal ischemia.

Materials and Methods. Focal cerebral ischemia was modeled by irreversible right-sided occlusion of the middle cerebral artery in male Wistar rats. The analyzed compound - 3-[(1E)-3-(3,4-dimethylphenyl)-3-oxoprope-1-en-1-yl]-4H-1-benzopyran-4-one and the reference – ethylmethylhydroxypyridine succinate were administered orally at doses of 50 mg/kg and 100 mg/kg, respectively, on for 3 days from the moment of ischemia modeling. After the specified time, a change in the size of the necrosis zone was assessed in rats, a change in the activity of enzymes- biomarkers of mitochondrial biogenesis – succinate dehydrogenase and cytochrome-c-oxidase, as well as the NOX4 content were determined in the mitochondrial fraction.

Results. The study showed that against the background of administration of ethylmethylhydroxypyridine succinate and 3-[(1E)-3-(3,4-dimethylphenyl)-3-oxoprope-1-en-1-yl]-4H-1-benzopyran-4-one to animals, a decrease in NOX4 content by 41.9% (p<0.05) and 42.8% (p<0.05) respectively was observed, which was accompanied by an increase in the activity of succinate dehydrogenase by 131.8% (p<0.05) and 137.5% (p<0.05), respectively, as well as cytochrome-c-oxidase by 83.1% (p<0.05) and 79.0% (p<0.05), respectively. It is worth noting that when using ethylmethylhydroxypyridine succinate and 3-[(1E)-3-(3,4-dimethylphenyl)-3-oxoprope-1-en-1-yl]-4H-1-benzopyran-4-oh, the brain necrosis zone in rats decreased by 17.6% (p<0.05) and 15.2% (p<0.05), respectively.

Conclusion. It has been shown that oral administration of 3-[(1E)-3-(3,4-dimethylphenyl)-3-oxoprope-1-en-1-yl]-4H-1-benzopyran-4-one to animals with focal ischemia leads to a decrease in the area of brain necrosis, which may be based on suppression NOX4 and, accordingly, improved mitochondrial function.

Introduction. The study focuses on the development of specialized nutrition for individuals with dysphagia using 3D printing technology. The relevance of the research and existing knowledge gaps are driven by the limited range of commercially available products for dysphagic patients, their high cost, and uniform texture, which negatively impact patients' quality of life. Existing studies explore various approaches to the development of 3D-printed food, including the use of protein isolates, starch-based gels, and stabilized purees; however, most of these approaches rely on expensive or hard-to-access components. 

Purpose. Analysis of nutritional recommendations for people with dysphagia and development of a tasty and attractive dish from natural ingredients, in order to meet the needs of medical institutions for products and ensure their availability for people with dysphagia.

Materials and Methods. The research methods included the formulation of food inks based on readily available domestic raw materials - potato, cabbage, carrot, onion, and zucchini powders - combined with guar and xanthan gums, experimental 3D printing, and texture evaluation according to IDDSI standards. 

Results. The results and discussion demonstrated that the developed product successfully passed the spoon tilt test, corresponding to Level 4 thickness, while the combination of thickeners ensured a homogeneous structure and shape stability for at least 30 minutes. Sensory evaluation identified the need for additional optimization of printing parameters to improve the product's appearance. Compared to previously published studies, this research demonstrated the feasibility of creating specialized nutrition with an adapted composition that meets the dietary requirements of dysphagic patients. 

Conclusions confirm the potential of 3D printing technology for medical nutrition, and future studies will focus on enhancing flavor characteristics, refining extrusion parameters, and conducting clinical validation of the developed product.

Introduction. Pectin is a biocompatible polysaccharide with multifunctional technological and biological activity, depending on its source and extraction method. Extracting pectin from by-products of juice or sugar production represents an approach to utilizing agro-industrial waste by obtaining a commercial product with high added value. Recently, there has been a growing trend to use pectin for producing edible coatings to protect food products, antimicrobial films based on biocomposites and nanoparticles, and for synthesizing various medicinal preparations and dosage forms. 

Purpose. The aim of this review is to analyze and summarize research findings regarding the relationship between the structural characteristics and functional properties of pectic polysaccharides.

Materials and Methods. The study is based on scientific publications, most of which were published between 2009 and 2024. The search and selection of articles were conducted using bibliographic databases such as eLIBRARY.RU, RSCI, Scopus, Web of Science, and PubMed. Data analysis involved systematization, summarization, intermediate conclusions, and a general conclusion utilizing elements of artificial intelligence.

Results. It has been concluded that due to the expanding applications of pectic polysaccharides and sources of pectin-containing raw materials, further research is necessary to investigate the relationship between the molecular-structural features of pectins, their functionality indicators, and their behavior in food and biomedical systems.

Introduction. Antimicrobial peptides and antibiotics produced by microorganisms are the leading group in pharmaceuticalл78 production in most economically developed countries. These drugs used in the treatment of various infectious diseases are one of the broadest categories. In Russia, antimicrobial agents are included in the list of vital and essential drugs. Currently, the problem of import substitution is acute due to the current political situation, so strategies for replacing imported pharmaceutical substances with domestic ones are currently being actively developed. Quantitative determination of the quality of antibiotic drugs is the main task for enterprises producing these drugs. The methods used for these tasks, such as biological, diffusion, turbidimetric, spectroscopic and chromatographic analyzes, require timely improvement and modifications to increase their specificity, sensitivity and reproducibility, which ultimately determines the quality of the drug. 

Purpose. The aim of this scientific study is to collect theoretical data and analyze the scientific literature to review the sector of antimicrobial peptides and antibiotics produced by microorganisms, especially glycopeptide antibiotics. 

Materials and Methods. Theoretical analysis, comparative analysis of the collected information, SWOT analysis; criterial method for analyzing information sources. Results and discussion. This study examined biological methods, their relevance for some antibiotics, such as glycopeptides, due to their complex chemical structure, which complicates the use of physicochemical analysis methods. Analysis of scientific publications in Russian and foreign (English) languages, reflecting the features of the characteristics of the glycopeptide antibiotic - vancomycin, its antimicrobial action, a block diagram of its production and microbial synthesis. When writing this review, peer-reviewed articles, patents and other sources published between 2019 and 2024, in Russian and English, were used. The search was conducted based on the following keywords: «pharmaceutical market», «antimicrobial peptides», «glycopeptide antibiotics», «vancomycin», «agar diffusion method», «turbidimetry method». As a result of the search for information sources, 50 publications posted in foreign and Russian-language databases (PubMed, Google scholar, E-library, Cyberleninka, State Pharmacopoeia of the Russian Federation) were selected. 

Results. The dynamics of the modern pharmaceutical market of the antibiotic sector was analyzed, problem areas were identified, antibiotics produced by microorganisms were considered and a comparative analysis with antimicrobial peptides was carried out. The characteristics of the main group of glycopeptide antibiotics studied in this review, in particular vancomycin, are given. Its mechanism of antimicrobial action, technological block diagram of production and microbial synthesis at the molecular level are described. Pharmacopoeial methods for determining antimicrobial activity, namely the agar diffusion method and the turbidimetric method, are considered, a comparative analysis of these methods is carried out, and positive and negative aspects of standardization and control of antibiotic activity are identified.